METHOD VALIDATION · LOD / LOQ

LOD / LOQ Calculator

Two estimation methods (Signal-to-Noise and Calibration Residuals), experimental LOQ confirmation, and back-calculation to sample units — matching the Battery Method Validation walkthrough.

Analyte & Instrument

Method 1 — Signal-to-Noise

S/N = peak height / (2 × RMS noise)

# Conc (µg/L) Peak (cps)
1
2
3
4
Conc (µg/L)Peak (cps)S/NFlag
0.010025.21.50below
0.020050.43.00≥ LOD
0.050016810.00≥ LOQ
0.10033620.00≥ LOQ

Interpolated Limits

LOD = 0.0200 µg/L  (S/N = 3:1)

LOQ = 0.0500 µg/L  (S/N = 10:1)

Method 2 — Calibration Residuals

LOD = 3.3σ/S  ·  LOQ = 10σ/S

Results

LOD = 3.3 × 18.5 / 3360

LOD = 0.0182 µg/L

LOQ = 10 × 18.5 / 3360

LOQ = 0.0551 µg/L

M1 LOQ = 0.0500 µg/L  ·  M2 LOQ = 0.0551 µg/L  ·  Methods consistent ✓

S/N vs Concentration — Cu (m/z 63, He-KED)

LOD 3:1 LOQ 10:1
LODLOQ00.02770.05540.08310.1110.004.609.2013.8018.4023.00Concentration (µg/L)S/N

LOQ Confirmation

n ≥ 6 reps at LOQ concentration · RSD ≤ 10% · recovery 80–120%

PASS

Replicate Values (µg/L)

n = 6
#C_found
1
2
3
4
5
6
#Found (µg/L)Recovery %
10.048096.00%
20.0510102.00%
30.049098.00%
40.0520104.00%
50.047094.00%
60.0500100.00%

n = 6    x̄ = 0.0495 µg/L

SD = 0.00187 µg/L

RSD = 3.78%  (≤ 10%)  ✓

Recovery = 99.00%  (80–120%)  ✓

LOQ in Sample Units

LOQsample (ppm) = LOQsol (µg/L) × V (mL) / (m (g) × 1000)

LOQ to use (µg/L)

0.0500 µg/L

Calculation Trace

LOQsol = 0.0500 µg/L

Dilution factor = 500.00 mL/g

= 0.0500 × 500.00 / 1000

LOQsample = 0.0250 ppm

= 0.50% of spec limit (5 ppm)  ✓ ≤ 10%

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